Preliminary Communication Lack of a Modulatory Effect of Imipramine on Glucocorticoid-induced Suppression of Interferon- and Interleukin-10 Production in Vitro

Antidepressant drugs have been shown to reverse some changes evoked by glucocorticoids or stress. In the present study we attempted to find out whether imipramine, one of the most frequently used antidepressant drugs, interfered with glucocorticoids, modulating the production of IFNand IL-10, pro-inflammatory and anti-inflammatory cytokines, respectively. We observed a significant inhibitory effect of hydrocortisone, dexamethasone and the glucocorticoid receptor agonist RU 28362, used at doses of 10 and 10 M, on the production of IFNand IL-10 by whole blood cells stimulated by mitogens. Imipramine at doses of 10 and 10 M did not modulate IFNor IL-10 production, whereas at a dose of 10 M it increased the production of IL-10 and decreased that of IFN, those results being statistically insignificant, though. A combination of imipramine and dexamethasone or hydrocortisone at doses of 10 or 10 M significantly suppressed the production of IFNand IL-10, the level of inhibition being similar to that observed for glucocorticoids alone. The classic antidepressant imipramine was not able to modulate the suppressive effect of “stress” doses of hydrocortisone on the production of cytokines.